Abstract Background: Infections caused by antibiotic resistance pose a serious global health threat, undermining our ability to treat common infections and deliver complex medical procedures. Antibiotic misuse, particularly in low-middle-income countries, is accelerating this problem. Aim of Study: The aim of this systematic review was to investigate the use of antibiotics and antibiotic resistance in dentistry. Patients and Methods: A systematic search was performed in PubMed, Google Scholar, Embase, the Cochrane library, and Web of Science, for studies that investigated the use of antibiotics and antibiotic resistance in dentistry. After extracting data, the pooled sensitivity, specificity, constructed summary receiver operating characteristics curve and positive likelihood ratio were estimated. 1436 abstracts were reviewed after duplication removal and subsequently the full text of the 110 articles was obtained according to the inclusion criteria. A total of 99 studies were excluded. Results: 12 studies were considered eligible after full-text review.The results revealed that there was significant improvement in homogeneity in subgroups classified by state, p-value, imaging techniques, and other factors (Chl2=148.60, p=<0.00001),I =93). Conclusions: The missuse of antibiotics contributes to the development of bacterial resistance. A proper use of antibiotics in treatment of dental, dental infections play a critical role in avoiding the emergence and transmission of resistance, and all healthcare personnel must be aware of this.