Abstract Background: OCT has evolved over the past decade as one of the most important ancillary tests in ophthalmic practice. OCT makes it possible to obtain noninvasive, rapid, objective, high-resolution, cross-sectional imaging of the retina, the (RNFL) and the optic nerve head and also permits direct, real-time imaging of ocular pathology that previously could not be visualized using traditional methods. Aim of Study: The purpose of this study is to evaluate the relationship between retinal nerve fiber layer thickness (RNFL) in myopia versus hypermetropia. Patients and Methods: A comparative study included a total of 68 eyes divided into 45 eyes with errors of refraction and 23 emmetropic eyes in the period from March 2020 to September 2020 (Myopic eyes above -4 D, hypermetropic eyes above +4D). Results: The results found in our study showed that there was statistically significant difference in RNFL thickness between myopic eyes, hypermetropic eyes in comparison to emmetropic eyes. RNFL thickness decreases in myopic eyes in the whole thickness and in all quadrants except in temporal quadrant, while RNFL thickness increases in hypermetropic eyes in the whole thickness and in all quadrants except in temporal quadrant. Conclusion: Our study is comparing the peripapillary RNFL thicknesses of myopic eyes versus hyperopic eyes. We have shown that peripapillary RNFL thickness differed with refractive status of the eye being thinner in myopic eyes and thicker in hyperopic eyes.