Abstract Background: Musculoskeletal pain is a common problem encountered in medical practice. Exercise canprovidet empo-rary relief from pain. Hypoalgesia is found to occur during and after single episodes of high intensity aerobic exercise in healthy adults. However, not all individuals are willing or able to engage in high-intensity aerobic exercise. Aim of Study: To examine the character of pain threshold modulation that occurs following short-duration isometric and dynamic exercises. Material and Methods: Eighty healthy female students of College of Applied Medical Sciences, King Saud University, age ranged between 18 and 23 years participated in study. The sample was divided randomly into two equal groups; first group was tested for the effect of isometric exercise on pressure pain threshold and the second group was tested for the effect of dynamic exercise on pain threshold. Results: Results indicated that there were significant effects for both isometric and dynamic exercises on pain threshold which was found to be elevated in both exercised and non-exercise dsides after isometric and dynamic exercises and lasted for ten minutes. Conclusion: It was concluded that short duration non-exhaustive isometric and dynamic exercises was associated with hypoalgesic responses in ipsilateral and contralateral sides in healthy young females.