Abstract Background: Computer Vision Syndrome (CVS) is an emerging health problem which is unceasingly increasing worldwide. It refers to a group of eye and vision problems experienced during or related to computer and other VDTs use. Aim of Study: The objectives were to determine the prevalence of CVS among students of Faculty of Medicine in Cairo University and to detect the relation of various factors in computer use with occurrence of symptoms. Patients and Methods: This study is a cross-section study in which 260 medical students from different grades of Faculty of Medicine in Cairo University were included. Participants were interviewed using self-administered Computer Vision Syndrome Questionnaire (CVS-Q). Results: The current study reveals that 75% of medical students in Faculty of Medicine suffered from CVS. Headache (81.5%) and eye pain (63.8%) are the most frequent CVS symptoms experienced by the students. CVS prevalence is found to be more among female students (78.7%) than among male ones (71%). It is also found to be more among glasses wearers (78.8%) than among non-glasses wearers (70.7%) and much more among contact lenses wearers (92.9%) than among non-contact lenses wearers (72.8%). There is significant linear correlation between duration of use of VDTs (by hours/ day) and the score of CVS. Conclusion: This study revealed that CVS is a prevalent problem among students of Faculty of Medicine, Cairo Uni-versity.