Aim: To evaluate the efficacy of different kinematics in removal of root canal filling materials. Methodology: A total of 60 human freshly extracted mature permanent maxillary central incisors were selected. Access cavities were prepared and the teeth were cleaned and shaped then obturated. Teeth were randomly divided into three groups according to the removal technique (manual H-file, ProTaper NEXT file and Reciproc file), then split longitudinally and photographed. The images were transferred to a computer, and total canal space and the remaining filling material were quantified. The ratio of the remaining filling material to the total root canal space was computed with the aid of AutoCAD software. The mean percentages of the remaining filling material and removal time using the Kruskal–Wallis, Friedman's test and one-way ANOVA test. Results: The mean percentage of the remaining filling material was significantly higher in group I than both II and III, While there is no statistically significant difference between group II and III. Regarding comparison between the root levels, the apical level showed the statistically significantly highest mean percentage of remaining endodontic filling material, followed by the middle and the coronal root levels. The time required to remove filling material was significantly shorter in group III followed by II and I. Conclusion: Total elimination of root canal filling is a challenge especially in the apical part. No statistically significant difference was found between the two kinematics regarding the efficiency in removing obturation materials. While reciprocation was faster than continuous rotation in removal time.