Abstract:
The aim of the study was to evaluate the effect of enamel matrix derivative and high ‎power diode laser irradiation on healing of periodontal ligament (PDL) of‎ experimentally avulsed and replanted upper incisors teeth of albino rats after ‎extended extra oral dry time through histological examination and immunohistochemical detection of fibronectin. Materials and methods: 30 adult male ‎Albino rats divided into three groups, 10 rats each; group I served as controls were no treatment for root surface of extracted incisors before their replantation, group II were root surface treated with enamel matrix derivative (Emdogain) gel, group III were root surface treated with diode laser and Emdogain gel. Five rats of each group were sacrificed four weeks from the beginning of the experiment while the other five rats were sacrificed after eight weeks. The jaws of the upper incisors regions of the sacrificed animals were dissected out for histological examination and immunohistochemical analysis. Results: The histological examination of group I rats showed massive degeneration and loss of orientation in the collagen fibers of PDL of replanted teeth, group II rats revealed signs of PDL regeneration in the form well-defined but unorganized fibers of PDL, while group III rats showed the best regenerative features in the form of well oriented and organized fibers of PDL. Immunohistochemical detection of fibronectin of group I showed negatively to weakly positive reaction. PDL of group II presented moderately positive reaction. While, group III presented strongly positive reaction.