Peripheral nerve injury (PNI) is a challenging injury in which healing relies on many factors. End-to-end neurorrhaphy (EEN) is the gold standard technique used in management of these injuries. Platelet rich fibrin (PRF) and low level laser therapy (LLLT) have the ability to enhance nerve regeneration after neurorrhaphy. The aim of this study is to evaluate the effect of LLLT as an adjunct to PRF use after end-to-end neurorrhaphy on sciatic nerve regeneration in rat model. Material and methods: This double-blind randomized study was performed on 20 Albino Wistar rats in the animal house of Misr University for Science and Technology. Animals were divided into two groups; group I in which nerve repair was performed using EEN with PRF membrane, in group II the same treatment was performed in addition to intraoperative application of LLLT diode laser. Within each group, animals were divided into two subgroups where rats were sacrificed after one and three weeks follow-up period. Assessment was performed using electromyography measurements and area percent evaluation of S100 protein immunostain at one and three weeks follow-up periods. Results: Comparison showed that electromyography measurements improved in group II more than group I with significant difference in the first week follow-up period. At three weeks follow-up period group II showed enhanced values than group I but with no significance. Histologic examination showed more enhanced regeneration with group II at both follow-up periods with significant difference. CONCLUSION: Application of LLLT enhances peripheral nerve regeneration of sciatic nerve in rat model.