Introduction: Creation of a sterile space is impossible in infected root canals, using mechanical preparation alone due to the complication configuration of radicular canal systems. Nearly half of the radicular canal dentine were left unprepared during the mechanical preparation with traditional stainless steel hand instruments and endodontic instruments. Aim; Evaluation of the outcome of numerous irrigating solutions used as final irrigation on the depth of sealer penetration into dentinal tubules. Methods: Two different endodontic sealers Bioceramic sealer and Resin sealer were used with four different irrigating solutions (Sodium hypochlorite, Chlorhexidine digluconate, Chiostan nanoparticles, Bio pure MTAD). GROUP 1: 5 ML of 2 % of were used as a final irrigant for 1 min. GROUP 2: 5 ML of 2% chlorhexidine were used as a final irrigant for 1min. GROUP 3: 5ML of 0.2% of chitosan nanoparticles were used as a final irrigant for 1 min. GROUP 4: 5ML of MTAD were used as a final irrigant for 1 min. Depth of sealer penetration into dentinal tubules was evaluated after samples preparation using confocal laser microscope analysis. Results: Comparison between different sealers of all irrigants in the coronal section was performed by using Independent T-test which revealed that MTA fill apex was significantly higher than AH plus as P < 0.05 in NaoCl. Conclusion: MTA Fill apex sealer exhibited a significantly greater dentinal penetration than AH plus sealer irrespective of the final irrigation protocol.