Introduction: 5-Flurouracil is considered the backbone of most of chemotherapy regimens, it has been established for the treatment of several neoplasms but one of its most common complications is oral mucositis including tongue epithelium.
Aim: This study aimed to explore the effect of Platelet rich plasma (PRP) against the cytotoxicity induced by 5-Flurouracil in albino rats' tongue.
Material & Methods: forty male Albino rats were used in this study, 10 rats were used as PRP donors and the other 30 rats were classified into 3 groups, 10 rats each: Group I: 60 mg/kg of saline was injected ip. on day zero, and other 40 mg/kg was injected on the second day. Group II: 60 mg/kg of 5-FU was injected ip. on day zero, and other 40 mg/kg was injected on the second day. Group III: 60 mg/kg of 5-FU was injected ip. on day zero, and other 40 mg/kg was injected on the second day then after 24 hours, 1ml/rat/day of PRP was injected locally into the tongue mucosa for 3 weeks. The animals were sacrificed after 1 week of the second injection in group I and II and at end of third week of PRP injection in group III. specimens were prepared for histological examination and immunohistochemical staining for CD3.
Results: Histological examination revealed significant improvement of mucosal tongue epithelium and connective tissue in group III samples when compared to group II, supported by results of CD3.
Conclusion: PRP has accelerating healing effect on tongue epithelium and connective tissue.