Objective: The purpose of this study was to evaluate the effect of different remineralization protocols: Fluoride mouth wash (Listerine total care zero), CPP-ACP, CPP-ACFP and CPP-ACP +Fluoride mouth wash on demineralized enamel surface micro hardness.
Materials and Methods: A total of thirty non carious freshly extracted human premolar orthodontic purpose were obtained as specimens and sections into two half were demineralized . The specimens were randomly divided into the following six treatment groups: (a): positive control,( b): negative control, (c): Listerine total care zero mouth wash, (d): CPP-ACP, (e): CPP-ACFP, (f): CPP-ACP +Listerine mouth wash. The testing procedures were used surface microhardness testing and environmental Scanning electron microscope.
Result: All remineralizing agents groups had the lowest statistically significant surface microhardness than control groups. The CPP-ACP + Listerine total care zero mouth wash showed a higher statically significant surface microhardness than the negative control.
Conclusion: Consistent with clinical findings, when applied only to demineralized teeth in this study, The CPP-ACP and CPP-ACPF should have a powerful effect in enhancing the surface structure and microhardness of demineralized enamel surface and External fluoride present in mouth wash has a more synergetic effect than the conjugated fluoride in CPP-ACPF in demineralized enamel surface remineralization.