Aim: To evaluate the antibacterial activity of Ibuprofen, Triple antibiotic paste(TAP) and Calcium hydroxide(CaOH2) used as intracanal medicaments against Enterococcus faecalis (E. faecalis) in single rooted teeth. Materials and methods: Seventy-five extracted single rooted teeth were decoronated, mechanically prepared up to F4 using Protaper universal rotary files, autoclaved, contaminated with E. faecalis and incubated at 37°C for 14 days. The teeth were then divided into 3 groups (25 each) according to the intracanal dressing used; Group 1: TAP, Group 2: Ibuprofen and a control group: Ca(OH)2. TAP and Ibuprofen powders were mixed with distilled water (1:1w/v), placed inside the canals while Ca(OH)2 was injected directly into the canals. The canals were then sealed and incubated at 37°C for 7 days. After 7 days the intracanal dressings were removed by irrigation. Bacterial samples were obtained from the canals using paper points before the application of the medicaments and the number of colony forming units (CFU) was calculated and recorded as (CFU1) then the same procedures were done after the intracanal medicaments application and removal to record (CFU2). The antibacterial action and the percentage of bacterial reduction for the tested medicaments were assessed. Results: Ibuprofen, TAP and Calcium hydroxide showed a significant antibacterial activity against E. faecalis (P<0.001). However, there was no statistically significant difference in the percentage of bacterial reduction between the Ibuprofen, the TAP and the calcium hydroxide groups against (P=0.270). Conclusion: Ibuprofen possess an antibacterial activity against Enterococcus faecalis which is comparable to that of TAP and calcium hydroxide.