Introduction: The aim of this study is to evaluate the effect of addition of silver nanoparticles (SNP) to AD Seal, MTA Fillapex and GuttaFlow 2 in terms of antibacterial effect. Methods: The method applied was the Direct Contact Test (DCT). Crushed sealers were put in sterile polyethylene tubes. About 18 mg of each sealer were weighed and placed in a tube. The tubes then incubated at 37 degrees Celsius then covered with 400 micro-L of Brain Heart Infusion (BHI) broth and 100 micro-L of bacterial suspension of Enterococcus Faecalis. After 1 min, 1 hour, the bacterial survival was evaluated by culturing on Brain Heart Infusion (BHI) agar plates and the Colony Forming Unit/mL (CFU/mL) were counted. Results: The results showed that there was no statistically significant difference between AD Seal and MTA Fillapex with or without addition of SNP at 1 min. However, a statistically significant difference was shown between GuttaFlow 2 and GuttaFlow 2 + SNP. At 1 hour, results showed a statistically significant difference between all groups except ADSeal + SNP and GuttaFlow 2 showed no statistically significant difference, with the highest antibacterial effect was for MTA Fillapex + SNP. There was statistically significant difference between all groups at 1 minute and 1 hour with the highest antibacterial effect at 1 hour except for AD Seal which showed the least antibacterial effect at 1 hour and the highest value favoring MTA Fillapex+ SNP. Conclusion: The addition of silver nanoparticles to all sealers increased their antibacterial effect.