The aim of this study was to compare marginal and internal adaptation of non-metallic implant supported crowns fabricated using split-file and scanning workflows. Materials and methods: A total of 30 crowns were fabricated and divided into two equal groups: Group I: where the master abutment was used virtually as a split-file workflow and Group II where the master abutment was scanned. Each group was subdivided into three equal subgroups according to material: subgroup A: zirconia, B: lithium disilicate, and C: polyetherketoneketone (PEKK). Margin and internal adaptation were evaluated using replica technique with a digital microscope. Results: Group II had lower marginal and internal gap means (71.55, 94.66 µm respectively) than Group I (85.44, 103.73 µm respectively). Zirconia had better margin and internal adaptation (69.27, 82.48 µm respectively) followed by PEKK (82.14, 105.4 µm respectively) then lithium disilicate which had the largest margin and internal gaps (84.08, 109.7 µm respectively). Conclusions: 1. Despite the better fit of the scanned abutment group, split-file technique has shown acceptable marginal and internal adaptation. 2. Milled zirconia showed better fit than PEKK and lithium disilicate, however the three materials had acceptable marginal and internal adaptation.