Surgical repair or neurorrhaphy of peripheral nerves is a major surgical and clinical challenge with disappointing results. End-to-end neurorrhaphy is a gold standard technique in nerve repair, many techniques have been developed to improve the healing of the nerve after surgical repair, one of these techniques is the use of Platelet Rich Fibrin (PRF) in conjunction with end-to-end neurorrhaphy. This study aims to evaluate the effect of platelet rich fibrin in enhancing nerve regeneration of the sciatic nerve after end-to-end neurorrhaphy in rat model. Material and methods: A double-blind randomized study was conducted on 20 Albino Wistar rats at the animal house of Misr University for Science and Technology, rats were divided into two groups, group I in which end-to-end neurorrhaphy was performed. In group II, after end-to-end neurorrhaphy, PRF in form of a membrane was used to wrap the area of neurorrhaphy. Preoperative and postoperative evaluation of nerve regeneration was performed at one and three weeks postoperatively using electromyography for nerve conduction measurements. Three parameters were used, the latency in conduction, the amplitude of voltage, and the reaction of degeneration postoperatively. Results: latency, amplitude, and the reaction of degeneration within each group showed improvement along follow-up periods with a significant difference. Comparison of results between the two groups showed a significant difference along the follow-up periods in favor of group II. Conclusion: PRF with end-to-end neurorrhaphy is superior to the end-to-end neurorrhaphy and is associated with improvement in nerve regeneration of sciatic nerve in rat model