Introduction: Preservation of pulp vitality is the principal objective in the management of exposed vital pulp of immature young permanent teeth. It has become challenging to identify the ideal pulp capping agent for each clinical case. Owing to the reported clinical and radiographic success of Aloe vera as a pulp capping material in primary teeth, it was tempting to investigate this material as a pulp medicament in young permanent teeth. Materials & Methods: Eight intact newly erupting immature young premolars, scheduled for extraction for orthodontic purposes, were selected for this pilot study. Two groups were implemented in the study; group I was applied Aloe vera gel as the pulpotomy material while group II received Portland Cement. Results: Histological sections of both studied groups showed well organized odontoblast-like cells with proximal nuclei and extended odontoblastic processes arranged near the orifices and along the peripheries of the radicular walls, accompanied by predentin layer and dentin with the classic arrangement of dentinal tubules. The gene expressions of IL-1β, TGF-β and DSPP were comparable between the Aloe vera and Portland cement group Conclusion: Based on the results of the current pilot study, Aloe vera gel proved comparable to the control group as a pulp capping material in pulpotomy of young permanent teeth.