Aim: This study was directed to assess the antibacterial activity and fluoride release potential of Alkasite material (Cention N) in comparison to two different bioactive materials (Activa BioACTIVE restorative and Zirconomer) at various time intervals Materials and methods: Total 75 disc-shaped specimens of three different restorative materials (Zirconomer, Activa BioACTIVE, and Cention N) were made. 45 specimens for fluoride release (n =15) and 30 specimens for antibacterial activity (n=10). For Antibacterial activity specimens divided into 3 groups (n=10) Group1: Zirconomer, Group2: Cention N and Group 3: Activea bioactive. For fluoride release the specimens divided into 3 groups (n =15) Group1: Zirconomer, Group2: Cention N and Group 3: Activea bioactive. Each fluoride release group was further subdivided into three equal subgroups (n=5) according to the storage time (one day, 7days and 14days). Antibacterial Activity and diameters of inhibition zone were recorded after 48 hours also Fluoride release was evaluated at the end of Day 1, 7and Day 14, using fluoride ion-selective electrode. Data analysis was performed using ANOVA and post-hoctest. Results: Zirconomer group revealed significantly the highest mean fluoride release value than in Cention N and Activea bioactive. Also, Cention N significantly higher fluoride release than Activa BioACTIVE. Cention N group showed statistically significant largest zone of inhibition against Streptococcus mutans at 48 hours followed by Zirconomer group, the smallest zone of inhibition observed with Activa bioactive group. Conclusion: Cention N and Zirconomer had better antibacterial activity and fluoride release while Activea bioactive had limited antibacterial activity and fluoride release.