Purpose: The purpose of this in vitro study was to investigate the effect of four solutions sterile water (control group), tea, coffee, cola on the color stability of different denture base materials (short cycle heat-polymerized PMMA, long cycle heat-polymerized PMMA, thermo plastic monomer free microcrystalline polymer and 3d printed PEEK).Material and methods: A total number of 160 specimens (forty specimens for each group) were constructed, specimens then were divided into four groups each group includes 10 from each denture base material (short cycle heat-polymerized PMMA, long cycle heat-polymerized PMMA, thermo plastic monomer free microcrystalline polymer, 3d printed PEEK denture base material) were stored in each solution in room temperature in a dark environment. Colorimetric measurements were done before immersion and 6 weeks after immersion. Color differences among specimens immersed in water (control group), and staining solutions were evaluated over time. Data were statistically analyzed, with one-way analysis of variance (ANOVA) (α = 0.05). ANOVA was followed by Duncan`s post hoc test to find which groups differed from each other.Results: Result showed significant difference between groups before and after immersion in different beverages except for Cola solution in the PEEK at P-value 0.05, control group had no change in color before and after immersion in sterile water. Tea and cola showed the highest change in color with thermo plastic monomer free microcrystalline polymer.