Continuous denture wearing, lack of adequate hygiene and infection with Candida albicans (C. albicans) are among the etiological factors of Denture stomatitis (DS). The management of DS ranges from the implementation of oral hygiene measures and removal of predisposing factors to the use of antifungals, depending on the severity of inflammation. Aim of the study: This study aimed to evaluate the short-term effect of heat killed lactobacillus acidophilus - Lacteol Fort® (LF)- as a probiotic and garlic extract -Tomex Plus® (TP)- as a prebiotic on salivary C. albicans counts and biofilm-forming ability, in elderly denture wearers with DS. Methods: This was a single-center, single‐blind, randomized pilot clinical trial. 27 complete denture wearers were randomly allocated into 3 groups; group I (control), group II (LF) and group III (TP). Each group was clinically assessed for DS and microbiologically evaluated for salivary C. albicans counts at baseline and after 15 days. The biofilm-forming ability of salivary C. albicans was assessed, as well, for each group at the end of the experimental period. Results: clinical assessment revealed a reduction in the total erythematous area dimension from 3.00 ± 0.40 mm for group II (LF) and from 3.20 ± 0.54 mm for group III (TP) to ≤2 mm for either group after treatment. Microbiological evaluation showed that C. albicans counts and biofilm-forming ability were significantly lowered in groups II (LF) and III (TP). Conclusion: LF and TP were both found to be effective in reducing DS associated inflammation, C. albicans counts and biofilm-forming ability.