Purpose: To evaluate Candida albicans adhesion and the surface roughness of polyamide denture base material coated with various concentrations of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer. Materials and Methods: A total of 80 disks polyamide resin specimens were fabricated and divided into 4 groups (n = 20/group). Surfaces of the specimens in three groups were coated with MPC in different concentrations (0.25 mol/L; 0.50 mol/L and 0.75 mol/L), while uncoated specimens were used as control. Surface roughness (SR) was evaluated using a profilometer. Specimens were incubated with C. albicans for 18 h and the number of adhered cells was determined. Statistically, the following tests were done: analysis of variance, Pearson correlation coefficient test, post hoc Tukey test. Results: Upon PMPC coating, surface roughness values were decreased, with a significant difference between different groups. A statistically significant decrease was observed in C. albicans adhesion in parallel with the increase in the MPC polymer concentration. There was no significant difference between 0.50 mol/L and 0.75 mol/L groups in terms of adhesion. These findings indicated that graft polymerization of MPC on polyamide denture base material reduces the adhesion of C. albicans, and may be evaluated as a coating for prevention of denture stomatitis. Conclusion: Coating of removable prosthesis with 0.75 mol/L MPC is suitable to reduce C. albicans adhesion on polyamide denture base.