Introduction: This study aimed to compare Guttaflow bioseal, MTA-Fillapex, and AH plus with regards to their adaptability on dentin surface. Methods: Thirty (n=30) single rooted human teeth were instrumented and divided into three groups according to the type of sealer used (each n=10). All root canals were obturated with lateral compaction technique. Roots were cut longitudinally and evaluated for adaptability using scanning electron microscope (SEM) at 1500x. Five readings were taken for the widest gap distances at the three thirds of the root for each specimen. Results: AH plus group showed less mean values of gap distances than the other groups at apical and middle two thirds, while GuttaFlow bioseal mean gap distance was less at the coronal one third. MTA-F group showed the higher mean values among the three thirds of the root with no significant difference. Conclusion: AH plus showed superior adaptability than GuttaFlow bioseal, while the worst was MTA-Fillapex.