Denture stomatitis is increased dramatically in the recent years, particularly in denture wearers. It is a common inflammatory reaction with multifactorial etiology that usually appears in the oral cavity as an erythematous reaction on the oral mucosa extends to the limits of the maxillary denture-bearing area. Candida albicans is regarded as the primary cause of denture-associated stomatitis owing to its high capability to adhere to oral tissues and denture surfaces in addition to biofilms formation. Candida species can interact with different microorganisms within the mouth resulting in a complex and mixed biofilm formation with an organized structure which is difficult to remove. The presence of bacteria in the acrylic biofilms resulted in increased pathogenicity and virulence of Candida biofilms making them more resistance to antifungal drugs. This article was introduced to highlight on the denture biofilm microbiota and its role in Candida albicans pathogenicity in addition to exploring the clinical manifestation, multifactorial etiology and management policies of Candida-associated denture stomatitis frequently faced in dental clinic.An online search of PubMed, Scopus, Science Direct, and Google Scholar was conducted using key words (Microbiota, Candida albicans, Denture stomatitis, Biofilm) from 1978 up to 2021. The management of denture stomatitis mainly depends on enhancement of oral hygiene processes, description of antifungal agents either topical or systemic, as well as disinfectants description. Recently, medical extracts that possess antimicrobial activity against oral pathogens was introduced as a safe and less side effects protocol for management of denture stomatitis.