Objective: To investigate microshear bond strength of bioactive restorative material to enamel and dentin bonded using universal adhesive in etch and rinse and self-etch mode stored for 24h and 6 m.
Methods: Sound bovine incisors were used in this study. ACITVA Bioactive restorative (Pulpdent Corp., Watertown, MA, USA) and Single Bond Universal adhesive (3M ESPE, St.Paul, MN, USA) were used. Teeth were grouped into 6 grouped according to bonding protocol (No bonding agent, bonding in etch and rinse mode and bonding in self-etch mode) and tooth substrate (enamel and Dentin). Specimens were stored for either 24 h or 6 m. Microshear bond strength (µSBS) were tested using universal testing machine and micromorphological observation of the interface were investigated using ESEM. Data were tabulated and statistically analyzed using Two-way ANOVA followed by Bonferroni's post-hoc test.
Results: Enamel bonded in etch and rinse mode recorded the highest µSBS values at both storage periods. Dentin at 24 h showed no significant difference between etch and rinse and self-etch modes while after 6 m, etch and rinse protocol showed significant drop. Control group recorded the lowest µSBS at all experimental groups.
Conclusion: Use of bonding agents is recommended with ACITVA to both enamel and dentin. Universal adhesives are preferred in etch & rinse mode with enamel and self-etch mode with dentin. ACITVA is able to preserve bond strength upon storage except for dentin bonded in etch and rinse mode.