Abstract

Introduction:
The aim of this study is evaluating marginal adaptation and sealing ability of nanoMTA and nanohydroxyapatite using bacterial microleakage method for sealing ability and scanning electron microscope (SEM) for marginal adaptation.

Methods:
0ne hundred mandibular premolar extracted teeth were used in this study then divided into two subgroups fifty teeth in each according to root-end filling materials used then further subdivided into twenty-five teeth in each according to the method of assessment. Teeth were prepared using M3 Pro Gold rotary files and obturated with single cone gutta-percha and resin sealer. The apical 3mm of the roots were resected. Root-end cavity preparation were prepared and filled with nanoMTA and Nanohydroxyapatite. Group 1: bacterial microleakage assessed to NanoMTA and nanohydroxyapatite by using a model for assessment of bacterial leakage by broth changed color from red (alkaline) to yellow (acidic) as a result of acid production from bacterial growth. The samples were monitored every 24 hours for a period of 30 days. Group2: Marginal gaps between root end filling materials NanoMTA and nanohydroxyapatite with root dentin walls observed under SEM. Data were collected and statically analyzed using ANOVA tests.

Results:
Samples shows insignificant statistical difference between the two materials in microleakage. However, statistically significant difference at marginal gaps in nano MTA samples and nanohydroxyapatite with better results with nano MTA retrograde filling material.

Conclusion:
Nanohydroxyapatite might be a promising mixture to be used as a root-end filling material, as it has about the same bacterial leakage results as nanoMTA