Background: Over the last few decades, several advancements have been made in caries prevention. The use of pit and fissure sealant is one of the essential forms of prevention. Sealants protect the occlusal surfaces, inhibiting bacterial growth and providing a smooth surface. Methods: Clinical study: Forty healthy children aged 6-10 years were selected from the outpatient clinic of the Pedodontic Department, Faculty of Dentistry, Tanta University. 40 molars were sealed with "Ionoseal" & 40 molars were sealed with "Fissurit F". Laboratory study: Twenty caries-free human permanent premolars were collected and stored in 0.5% chloramines solution, then cleaned and stored in distilled water (20°C) until use. Shear bond strength was measured using a universal testing machine at cross-head speed of 1mm/min. Results: For the clinical study, there was statistically significant difference between the two groups but the quality of restorations gradually decreased from 3-months visit to 9 months visit in both groups. "Fissurit F" had better clinical performance than "Ionoseal". Both materials showed similar results with respect to caries prevention. The bond strength of "Ionseal" was statistically lower than "Fissurit F". Conclusion: The resin based fissure sealant had better clinical performance than glass ionomer cement. Both materials showed similar results with respect to caries prevention