Aim: To compare push-out bond strength of gutta percha and three root canal sealers AH-Plus, MTA Fillapex, Gutta flow 2. Materials & Methods: Sixty human freshly extracted lower premolars were prepared using nickel titanium Revo-S files till AS 40 finishing file and divided into three equal groups n=20, Group I: Gutta percha/AH-Plus sealer, Group II: Gutta percha/ MTA Fillapex, Group III: Gutta percha/Gutta flow 2. Each root was sectioned in a horizontal plane, from each root three sections with 2 mm in thickness were obtained at 1, 4, 9 mm from apex representing apical, middle, and coronal respectively. The push-out bond strength was determined for each root slice using universal testing machine. Data were analyzed using one-way ANOVA test. Results: AH-Plus showed the highest bond strength while the Gutta flow 2 showed the lowest, there was no statistically significant difference between the three levels apical, middle & coronal. Conclusions: The composition of endodontic sealers affects their push out bond strength, where the higher push out bond strength was found in GP/AH Plus group and the lowest in gutta flow 2, while there was insignificant difference in adhesion within the tested groups at different levels.