Statement of the problem. Selection of the proper prosthetic restorative material is still a dilemma facing most of the clinicians. Purpose. The aim of this study was to evaluate the effect of different framework materials on the stress distribution in a posterior three-unit fixed dental prosthesis. Materials and methods. One 3D finite element model was specially prepared by scanning a model of a fixed dental prosthesis (FDP) in the posterior region. The model had missing maxillary first bicuspid and prepared abutments, that representing maxillary cuspid and second bicuspid. The model was simulated as restored by three different materials; Nickel-Chromium (Ni-Cr), Zirconia and Bio-HPP (PEEK). The model was subjected to compressive vertical load of 200N applied at the central fossa of the pontic. Von Mises stress values were determined. Results. Three linear static analyses were carried out. Stress and deformation fields generated under applied load were compared. Location of both maximum Von Mises stress and maximum total deformation were found on the abutment finish line towards the pontic. Conclusion. Special attention should be considered to the finish line contact with fixed dental prosthesis to avoid stress concentration. Tooth may be insensitive to fixed dental prosthesis materials