Objectives: To evaluate the antimicrobial activity and adhesive properties of a simplified totaletch
adhesive system containing different proportions of Hesperidin (HPN).
Materials and Methods: Hesperidin was added to dental adhesive in three different ratios
producing four experimental adhesive groups (0 [control], 0.2, 0.5, and 1%). The antibacterial
activity of the prepared adhesive groups was studied using agar disc-diffusion test against
Streptococcus mutans. The viscosity of dental adhesives was evaluated using a cone and plate
viscometer. Microtensile bond strength was tested immediately and after thermocycling.
The fracture patterns were examined using a stereomicroscope. Data were statistically analyzed
using ANOVA and Tukey HSD tests (α = 0.05).
Results: The antimicrobial activity of HPN-incorporated experimental adhesives exhibited
a significant inhibitory effect against Streptococcus mutans compared with the control (P < 0.05).
The viscosity of the experimental adhesives increased with increasing the concentrations of HPN
incorporation into the adhesive. The incorporation of 0.2 wt% and 0.5 wt% HPN into the dental
adhesive significantly increased the immediate μTBS (P < 0.05). However, experimental adhesives
incorporating 1 wt% HPN showed no significant differences in the μTBS values compared with the
unmodified adhesive resin (P > 0.05). After thermocycling, all studied adhesive groups revealed
significant reduction in μTBS (p < 0.001).
Conclusions: 0.5 wt% HPN incorporated dental adhesives could achieve a promising
antibacterial effect without adversely affect the adhesive characteristics; however, thermocycling
significantly reduced the μTBS.