Aim: To compare the efficacy of chicken egg shell solution powder (CESP) and amorphous
calcium phosphate (ACP) on the remineralization of enamel subsurface lesions.
Materials and Methods: Twenty bovine central incisor teeth were used in this study. The
microhardness and the minerals content of all specimens were initially assessed using Vickers
hardness tester and EDXA respectively (positive control group).The specimens were artificially
demineralized and then reassessed directly after demineralization (negative control group). All
demineralized specimens were randomly classified into 2 equal groups,group I :treated with Chicken
eggshell powder solution (10 specimens), while, groupII: treated with ACP (10 specimens). The
remineralizing agent were applied twice daily for 15 min each for seven successive days. Finally all
specimens were reassessed for minerals content (Ca and PO4 weight %) and surface microhardness.
The data were statistically analyzed using repeated measures analysis of variance (ANOVA ), the
significance level was set at P ≤ 0.05.
Results: Microhardness was significantly decreased in all specimens after demineralization
and then it was significantly increased after exposure to therapeutic solutions. There was a
significant increase (P<0.001) in both Calcium and Phosphorus levels after remineralization using
both agents, as detected by EDAX. However, there was no significant difference between the two
remineralized groups.
Conclusions: Within the limitations of this in vitro study, it can be concluded that both
remineralizing agents were similarly able to increase the microhardness and tooth remineralization.
However, being natural products, CESP can be considered as an optimal alternative to the
commercial ones.