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76803

Effect of fish oil supplementation on alveolar bone structure in rats with glucocorticoid induced osteoporosis (Histological, immunohistochemical & ultrastructural study)

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Last updated: 22 Jan 2023

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Abstract

Glucocorticoids are widely used in treatment of many medical disorders. However, many side effects are associated with the chronic use of glucocorticoids administration especially osteoporosis. Fish oil contains omega 3 polyunsaturated fatty acids which can inhibit the production of inflammatory mediators such as IL-6 and TNF-α which are important for osteoclastogenesis. So the aim of the present work was to test the efficiency of fish oil administration on alveolar bone osteoporosis induced by glucocorticoid administration. Materials & methods: 8 Wistar albino rats were divided into 3 groups. Group Ι: control group. Group ΙΙ (osteoporotic group): rats were given 7 mg/kg of dexamethasone, once a week intramuscularly for 5 weeks. Group ΙΙΙ (fish oil treated group): rats were given the same dose of dexamethasone like group ΙΙ and at the same time were given daily an oral dose of fish oil 1gm/kg/day. All rats were sacrificed after 5 weeks from the start of the experiment and molar segments of the jaw were dissected out. They were examined histologically, immunohistochemically, histomorphometrically and ultrastructurally. Results: Histologically in osteoporotic group ΙΙ the bone showed marked resorption with the presence of many osteoclasts which showed significant increase in number and intense TRAP immunostaining. By scanning electron microscope the buccal cortical plate showed obvious erosion. On the other hand, fish oil treated group showed marked improvement where the bone appeared denser with significant decrease in osteoclast number which exhibited mild TRAP staining. Histomorphometriclly, the bone surface area showed marked increase. Ultrastructurally, the bone surface appeared relatively smooth. Conclusions: fish oil supplementation could to a large extent overcome the detrimental effect of glucocorticoid administration on the structure of the alveolar bone.

DOI

10.21608/edj.2018.76803

Keywords

fish oil, Osteoporosis, osteoclasts, alveolar bone

Authors

First Name

Dina

Last Name

Nagui

MiddleName

Ali

Affiliation

Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.

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Orcid

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First Name

Nesma

Last Name

Khalil

MiddleName

Mohamed

Affiliation

Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.

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Volume

64

Article Issue

Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)

Related Issue

11453

Issue Date

2018-07-01

Receive Date

2020-03-10

Publish Date

2018-07-01

Page Start

2,305

Page End

2,317

Print ISSN

0070-9484

Online ISSN

2090-2360

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https://edj.journals.ekb.eg/article_76803.html

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https://edj.journals.ekb.eg/service?article_code=76803

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21

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Original Article

Type Code

254

Publication Type

Journal

Publication Title

Egyptian Dental Journal

Publication Link

https://edj.journals.ekb.eg/

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Article

Created At

22 Jan 2023