Surgical management of ameloblastomas depends on radiographic extension of the lesion and the histological findings up till now. Meanwhile, there is controversy regarding the surgical techniques of different types of ameloblastomas. The aim of this study was to assess the degree of lesion aggressiveness, using p53, to determine the potential biologic behavior of those lesions. Patients and Methods, were conducted on 17 patients (10 males and 7 females), aged from 13 to 67 year. These patients divided initially as primary, recurrent, and of malignant ameloblastoma. Immunohistochemical analysis was used to classify ameloblastomas into aggressive and non-aggressive lesions. Surgical technique was done according the immunohistochemical result. Postoperative results were accepted for  patients and no recurrence was observed after surgical excision in all cases.  The present study concluded that p53 is good approach to detect mutation and the biological behavior of the cells upon which surgery should be addressed. This study provided that treatment strategy of the ameloblastoma must be preceded by immunohistochemical evaluation to detect the natural behavior of the cells. Moreover, the conventional histological and radiographical assessment is not enough for the final management of such cases.