Objectives: Replantation of avulsed tooth depends on the viability of periodontal ligament cells. The aim of this study was to evaluate the survival of periodontal ligament cells (PDL) when placed in platelet-rich fibrin (PRF) transport media after an extra-oral dry time of 40 minutes.
Method: Forty human mandibular first premolar teeth with healthy periodontium and closed apices, previously planned for orthodontic extraction, were selected. The teeth were randomly divided into study group, PRF and control group, Hank‘s balanced salt solution (HBSS) as a reference medium. In both groups, the teeth were stored dry for 40 minutes, followed by 30 minutes immersion in the study and control media.
Results: There is a statistically significant increase in the number of viable PDL cells in PRF group as compared to reference medium group.
Conclusion: PRF demonstrated higher number of viable PDL cells and hence could be a suitable transport medium for avulsed teeth.