Aim: The aim of this study was to compare the cytotoxicity of triple antibiotic paste, calcium hydroxide and sodium hypochlorite gel when used as intra-canal medicaments on cultured fibroblasts.
Methodology: Experimental groups were divided into three groups; Group (CaOH), Group (TAP) & Group (NaOCl). Cytotoxicity of tested materials was determined by the microculture tetrazolium (MTT) assay. Optical density representing the residual living cells was determined for each group at different concentrations at 2h, 4h, 24h and 48h. Data were then collected, tabulated and statistically analyzed
Results: Statistical analysis showed that the duration range from 2 hours up to 48 hours has no significant effect for each of the three tested medicaments. There was no significant difference between CaOH and NaOCl groups wheareas TAP group recorded significantly lower viability results.
Conclusion: Under the condition of the current study, NaOCl gel is considered acceptable intracanal medicament regarding cytotoxicity in comparison to CaOH and Triple antibiotic paste.