Objectives: The aim of this study was to evaluate the microhardness of root dentin wall before and after irrigation with BioPure MTAD, 17% Ethylenediamine tetra acetic acid (EDTA) and sodium hypochlorite (NaOCl).
Study Design: Sixty-four freshly human extracted single-rooted teeth was used in this study. The teeth were divided into 4 groups, according to the irriganting solution used as final rinse: group (1) BioPure MTAD, group (2) 17% EDTA, group (3) 5.25% NaOCL and group (4) control group (distilled water). The teeth were sectioned longitudinally and prepared for evaluation of microhardness of root dentine before and after irrigation with each irrigants which the baseline microhardness testing was carried out using Vickers Microhardness Tester.
Results: The results were statistically analyzed (p<0.05) showing significant difference between all the irriganting solutions in relation to the control group.
Conclusion: The EDTA and BioPure MTAD drastically reduced the microhardness of root canal dentin followed by NaOCL.