Beta
75018

HISTOLOGICAL AND ULTRASTRUCTURAL EFFECT OF ZINC SULPHATE SUPPLEMENTATION ON THE ALVEOLAR BONE OF CALCIUM DEFICIENT RATS

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

-

Abstract

Objective: The aim of the present study was to investigate the efficiency of zinc sulphate supplementation in treatment of the alveolar bone osteoporosis in rats induced by calcium
deficient diet.
Design: Thirty adult male Wistar rats with an average weight of 200-250 grams were randomized into 3 groups. Group Ι (control group): received standard rat chow, Group ΙΙ (osteoporotic group): received calcium deficient diet and Group ΙΙΙ (zinc treated group): received calcium deficient diet plus oral supplementation of zinc sulphate. After 3 months, the rats were sacrificed. The mandibles were examined histologically, histomorphometrically, ultrastructurally and by energy dispersive
X ray microanalysis (EDX).
Results: Histologically the impaired bone quality induced by calcium deficiency were significantly improved by zinc supplementation. The alveolar bone restored its structural organization in form of thick bone trabeculae, decrease number of osteoclasts and regularly distributed osteocytes. Histomorphometric analysis showed significant reduction in area percentage of alveolar bone trabeculae in osteoporotic group (p<0.001), while zinc treated group showed significant increase in the area percentage of bone compared to the osteoporotic group (p<0.001). Ultrastructurally, surface roughness and irregularity of the buccal cortical plate were observed in osteoporotic group. However, zinc treated group showed smooth and regular bone surface with minor irregularities. EDX microanalysis indicated a significant decrease in calcium content in osteoporotic group (p<0.001), while zinc treated group showed significant increase in the calcium percentage of bone compared to the osteoporotic group (p<0.001).
Conclusions: In a rat model zinc supplementation prevents the bone loss induced by calcium deficient diet.

DOI

10.21608/edj.2017.75018

Keywords

zinc, alveolar bone, Osteoporosis, Calcium deficiency

Authors

First Name

Dina

Last Name

Nagui

MiddleName

Ali

Affiliation

Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

Nesma

Last Name

Khalil

MiddleName

Mohamed

Affiliation

Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

Volume

63

Article Issue

Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)

Related Issue

11144

Issue Date

2017-01-01

Receive Date

2020-03-02

Publish Date

2017-01-01

Page Start

699

Page End

710

Print ISSN

0070-9484

Online ISSN

2090-2360

Link

https://edj.journals.ekb.eg/article_75018.html

Detail API

https://edj.journals.ekb.eg/service?article_code=75018

Order

24

Type

Original Article

Type Code

254

Publication Type

Journal

Publication Title

Egyptian Dental Journal

Publication Link

https://edj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023