Objective: to compare the shear bond strength of resin composite using universal adhesive   system containing MDP and resin modified glass ionomer cement to cementum and compare the results with those of cervical dentin. Materials and methods: A total of 12 lower premolars were used in this study, they were sectioned just above the cemento-enamel junction, the roots were then sectioned into two halves and divided into two main groups according to the tooth substrate in which group 1) Cervical dentin and group 2) Cementum. They were further subdivided into two subgroups according to the material used where n= 6  subgroup 1) Resin composite (Brilliant Ever-Glow) using universal adhesive (One coat 7 universal), subgroup 2) Resin modified glass ionomer (Fuji II LC). Shear bond strength was then evaluated for all specimens and statistically analyzed. Results: There was statistically significant difference between resin composite using universal adhesive system and resin modified glass ionomer when applied on cementum. While on comparing the resin composite using universal adhesive system and resin modified glass ionomer on cervical dentin it showed no statistically significant difference. Meanwhile no statistically significant difference was found between cervical dentin and cementum when using resin composite with universal adhesive system. Comparing resin modified glass ionomer on dentin and cementum there was also no statistical difference. Conclusion: using universal adhesive containing MDP with resin composite it showed the same bond strength to cervical dentin and cementum. While the resin modified glass ionomer showed higher bond strength to cementum than that of the universal adhesive system.