This study aimed to compare the antimicrobial effect of Nano-Silver Irrigant, Sodium Hypochlorite and Chlorohexidine against Enterococcus Faecalis bifilm. 60 Sixty, recently extracted, sound maxillary anterior teeth with completely formed apices and straight roots were selected in this study. Canals were instrumented using the Protaper Next system (Dentsply Sirona) 1 mm shorter from the apical foramen up to size X5 ( size# 50/06).  Sterility of the teeth after complete root canal cleaning and shaping were achieved by autoclaving for 15 min at 121ºC with the teeth immersed in distilled water. Enterococcus Faecalis Biofilm was prepared by mixing 5 mL of the bacterial inoculum with sterilized BHI, then inoculated in teeth samples for 60 days. Teeth were then divided after the incubation period into three groups; 20 teeth each, and were treated by delivering the each of the tested irrigating solution using sterile plastic syringes. The first group was treated with Nano-Silver 0.02mol/L (2000ppm) while the second was treated using with Sodium Hypochlorite (NaOCl) 5.25% and the third was treated usinwith Chlorohexidine (CHX) 2.0%. Serial rinses ensured 5 minutes contact period between the aseptic solution and the bacteria. Sterile paper points size 50 were introduced into the canals and maintained for 3 min for sample collection, after which the paper points were placed in test tubes containing 2ml of sterile saline and several dilutions were prepared. 100 µL of each dilution was applied to the blood agar culture plates and incubated at 37ºC for 24 hours. A classical bacterial counting technique was used for each group after the treatment application for the recovery of viable E. faecalis on agar plates The results showed that the NaOCl had the highest antibacterial activity with high statistically significant difference with the other groups followed by the chlorhexidine group which also show statistically significant difference than the other two groups, then came the nano silver irrigant group which showed the lowest antibacterial activity among all the groups with statistically significant difference with them.