The ability of reduced graphene oxide (rGo) in regenerating bone tissue has recently gained significant attention in biomedicine. Aim: we investigated the effects of rGo nanoparticles and platelet rich plasma (PRP) in regenerating induced defects in irradiated 2 types of rat bones. Materials and Methods: We had 4 experimental rat groups, each was 6 in number, all of them were irradiated then a bone defect was surgically induced at the condyle representing endochondral bone and ramus representing intramembranous bone in each rat. The bone defect was then grafted with rGo alone in (GpI), PRP alone in GpII, combination of PRP and rGO in GPIII or left ungrafted as a control in GpIV. 8 weeks later samples were explanted for histological studies. Results: All groups showed bone formation with highest organized bone formation in GPIII. Conclusion: regeneration of irradiated bony defects using the combination of rGO nanoparticles and PRP can provide the highest active healthy bone formation.