This study aimed to evaluate one-visit apexification with MTA apical plug versus traditional calcium hydroxide apexification with the help of platelet rich fibrin (PRF) apical matrix. Methods: 18 immature permanent maxillary anterior teeth with non-vital pulp were chosen from 14 children ranging from 9 to15 years old, and were divided randomly into two main groups with 9 teeth in each group [Group I MTA, and Group 2  Ca(OH)2]. Platelet Rich Fibrin was used for both groups as internal apical matrix. In group 1 MTA approximately 4-5 mm thickness of mixed MTA apical plug was placed at the apical end of the root, then the canal was obturated by Obtura II system after 24 hours, while in group 2 Ca(OH)2 ; the apexification was done by Ca(OH)2 and renewed at 3,6 months and after 12 months the canal was obturated by Obtura II system. The radiographs were taken for all cases at preoperatively, post operatively and at 3, 6 and 12 months follow up. Data were subjected to statistically analyze. Results: At 12 months follow up period 100% of teeth with calcific apical barrier were detected in Group 1 MTA, and 88% of teeth with calcific apical barrier were detected in Group 2  Ca (OH)2. However, there was no difference in statistically point of view between the groups. Conclusions: One-visit apexification provides an alternative treatment option over the traditional calcium hydroxide apexification in treating the non-vital cases with open apices.