Background: Thousands of people die every day around the world from infections acquired while receiving health care. Infection control encompasses the processes and activities that identify and reduce the risks of acquiring and transmitting endemic or epidemic infections among individuals. Aim of the study: The aim is assessment of infection control measures at the primary health care units in Assiut Governorate. Materials and Methods: The present study is a cross-sectional study that had been conducted in three districts out of the eleven districts composing Assiut Governorate. The three districts had been randomly selected and they are Assiut District (include urban and rural areas), Al-fat-h District and Sedfa District. The study covered all the primary health care units located in these three districts during the period from August 2013 to January 2014. Data were analyzed using SPSS version 16. Results: This study totally covered the 79 primary health care units and it was found that 94.9% of the units their physicians were trained on infection control program, while all nurses were trained on infection control. As a total, Rural Assiut had the highest mean percentage (53.52 ± 5.33). The highest mean percentage in administrative measures was found in West Assiut Neighborhood Administration (64.98 ± 6.09). Conclusion and Recommendations: There are statistically significant differences among the studied areas in all aspects of infection control as general, rural Assiut had the highest mean percentage. Continuous supervision and take an action against any person does not follow these procedures will help in rise the level of infection control in primary health care units in Assiut Governorate.