ABSTRACT
Background: The bone marrow has been considered as a main source for isolation of multipotent mesenchymal stem cells (MSCs). They are still the most frequently investigated cell type and often identified as the gold standard. However, a similar cell population has been also isolated from other tissues such as adipose tissue. Unlike bone marrow, the adipose tissue is abundantly accessible source of stem cell that can give a good yield in culture.
Aim of the work: Was isolation of the rat bone marrow MSCs (BM-MSCs) and adipose tissue MSCs (AD-MSCs) and assessing their growth kinetics.
Material and Methods: The rat bone marrow and adipose tissue were isolated from 10 male adult albino rats and cultured and expanded through 6 passages. BM-MSCs and AD-MSCs biological characteristics evaluated for cell therapy (morphology, flow cytometric analysis, colony-forming unit-fibroblast assay, proliferation capacity at passages 2, 4 and 6, population doubling time (PDT) and cell growth curves).
Results: BM-MSCs and AD-MSCs attached to the culture flask and displayed spindle-shaped morphology, more evident in AD-MSCs. Proliferation rate of AD-MSCs in the analyzed passages was more than BM-MSCs. The increase in the PDT of both types of MSCs occurred with the increase in the number of passages.
Conclusion: The rat AD- MSCs have growth kinetic advantages in the proliferative capacity, colony-forming unite fibroblast and population doubling time more than that of BM-MSCs. these advantages should be considered when choosing a stem cell source for specific clinical application.