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26455

Effect of Garlic on Rat Diabetic Renal Cortex. A histological and Immunohistochemical Study

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Last updated: 22 Jan 2023

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Abstract

Background: Diabetic nephropathy is one of the chief microvascular complications of diabetes and it has turn into the main cause of end-stage renal disease nictitate dialysis.
Aim of work: To observe the effects of garlic supplementation on STZ induced diabetic nephropathy in Albino rats using histological and immunohistochemichal stains.
Material and Methods: Three groups were included, control group of 5 rats served as control, experimental group of streptozotocin- induced diabetes with dose of 50 mg/kg body weight intraperitoneal for eight weeks and third group is the diabetic rats supplemented with garlic orally with a dose of (500 mg/kg body weight) for 6 weeks starting two weeks after diabetes induction.
At the end of the experiment, rats were sacrificed then kidneys were dissected out and processed for paraffin blocks. Sections were stained with haematoxylin & eosin and immunohistochemistry for vimentin, nuclear factor kappa and desmin.
Results: Increased diameter of glumeruli in diabetic rats. These changes were decreased in the diabetic group treated with garlic. Desmin, vimentin and NF-κB expression were induced in diabetic group but they decreased after garlic supplementation.
Conclusion: The changes in diabetic group were regressed with garlic supplementation. It is advisable to give natural products like garlic to diabetic patients as they help to ameliorate diabetic complications.

DOI

10.21608/EJANA.2018.16904

Keywords

Desmin, diabetic nephropathy, Garlic, nuclear factor kappa, vimentin

Authors

First Name

Omnia

Last Name

Erfan

MiddleName

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Affiliation

Anatomy Department, Faculty of Medicine, Mansoura University, Egypt

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Volume

40

Article Issue

2

Related Issue

4760

Issue Date

2017-07-01

Receive Date

2019-02-04

Publish Date

2017-07-01

Page Start

253

Page End

264

Print ISSN

1110-2144

Online ISSN

2090-259X

Link

https://ejana.journals.ekb.eg/article_26455.html

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https://ejana.journals.ekb.eg/service?article_code=26455

Order

3

Type

Original Article

Type Code

231

Publication Type

Journal

Publication Title

The Egyptian Journal of Anatomy

Publication Link

https://ejana.journals.ekb.eg/

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Article

Created At

22 Jan 2023