This paper was described antibiogram and heavy metal resistance pattern of Vibrio alginolyticus isolated from Asian Seabass (Lates calcarifer) hatchery. V. alginolyticus was recognized as a causative agent of vibriosis in Asian seabass and may lead to huge loss to the fish farmer. Therefore, this study was carried out to investigate the suitable of antibiotic to against V. alginolyticus. In the present study, a total of 14 antibiotics; oxolinic acid (2μg), ampicillin (10μg), erythromycin (15μg), furazolidone (15μg), lincomycin (15μg), colistin sulphate (25μg), oleandomycin (15μg), doxycycline (30μg), fosfomycin (50μg), florfenicol (30μg), flumequine (30μg), tetracycline (30μg), fosfomycin (50μg) and spiramycin (100μg) as well as four heavy metals; mercury (Hg2+), cadmium (Cd2+), chromium (Cr6+) and copper (Cu2+) were applied in the present study. Based on the antibiotic sensitivity test result showed that oxolinic acid the most effective antibiotic in controlling V. alginolyticus in which 93.3% of the present bacterial isolates were sensitive to it. This was followed by furazolidone (86.7%), nitrofuratoin (80.0%), tetracycline (73.3%), doxycycline (73.3%) and florfenicol (73.3%). On the other hand, all the present bacterial isolates were resistant to lincomycin. In the heavy metal tolerance test, all the present bacterial isolates were resistant to Hg2+, Cd2+, Cr6+ whereas only 26.7% of them were resistant to Cu2+.