The objective of this study was recorded the most prevailing bacterial agents which spreads in the cultured fish in Ismailia province, Eastern Egypt. This study was carried out on 1080 premature fish. 360 Dicentrarchus labrax (225±25 g) and 360 Sparus aurata  (150±25 g), and 360 Mugil cephalus (125±25). The examined fishes showed exophthalmia, hemorrhagic area on the skin, around the buccal cavity, head, and on the base of the fines, ulcers varied in their degrees, dark colour, and respiratory signs. The recorded postmortem lesions were septicemic signs with enlargement and congestion of the liver and spleen with distended gall bladder. With the presence of serous to hemorrhagic ascetic fluid, the stomach and intestines displayed congestion, thickening, and inflammation of their walls. The total prevalence of bacterial infection in examined premature fishes was 27.68%. The highest percentage was in D. labrax at 38.88%, followed by S. aurata at 27.22%, and the lowest percentage in M. cephalus at 16.94%. The most prevailing bacterial agents were Vibrio spp and Pseudomonas spp.