The current designed to investigate the role of Magnesium Oxide Nanoparticles in healing process of cervical esophageal anastomosis in dogs. An Adults eighteen healthy stray dogs of both sexes were allocated equally in to two groups 9 of each, each main group was Moreover ,divided into 3 subgroups of 3 dogs in each according to the period post-surgery at 7,15,30 days . All animals underwent to end - end cervical esophageal anastomosis and the mucosal layer was sealed 2/0 silk with a simple interrupted pattern and the knot tied within the lumen, Whereas, the muscular layer was closed with 2/0 polygalactin (Vicryl) in an interrupted horizontal mattress, finally the neck muscles were closed routinely. In group one G1 (control group) the mucosa and muscularis layers at anastomotic site were sutured only without treatment, while, in group two G2 (MgO NPs) were treated by spreading of MgO NPs. The result of the study showed that the healing process were superior with the MgO NPs group compared to control group significantly at P < 0.05, based up on both histological and gross pathological observations. this study conclude that local spreading of MgO NPs was accelerate healing of esophageal anastomosis and promote return the tissue to their Furniture and function pre surgery