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The Effect of Autologous and Homologous Platelets-Rich Plasma Gel on Cutaneous Wound Healing In Rescued Donkeys: A Comparative Study

Article

Last updated: 01 Jan 2025

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Tags

Veterinary Pathology & Animal Diseases

Abstract

Background: Cutaneous wound healing in equine is frequently delayed and complicated. Therefore, a new therapy is needed to achieve rapid and satisfactory healing. Aims: This study aimed to investigate whether the use of autologous PRP gel in cutaneous wounds enhances wound repair and alters oxidative stress cascades than homologous PRP-gel in the rescued donkeys. Methods: 10 rescued donkeys were selected and allocated into two groups A and B. Group-A donkeys (n=5) were treated with APRP-gel, while group-B donkeys (n=5) were treated with HPRP-gel. A full-thickness (4 mm2) skin wounds were selected in each donkey. Wound re-epithelialization was assessed by H&E staining and collagen re-establishment was assessed by Masson's trichrome staining. The CAT activity and MDA concentration were assessed in blood samples on days 7, 21, and 36. Results: We observed a significant increase in re-epithelialization, fibroblasts and angiogenesis from days 21 (P < 0.05) to 36 (P < 0.01) in APRP-wounds than HPRP-wounds. Collagen organization in APRP-wounds was good (P<0.05) from days 21 to 36 than HPRP-wounds. Malondialdehyde (MDA) concentrations in APRP-wounds were significantly decreased from day 21 to day 36 compared with HPRP-wounds (P<0.01). There was no difference in catalase (CAT) activity between APRP and HPRP wounds (P>0.05). Conclusion: APRP-wounds improved cutaneous wound healing in rescued donkeys by reducing oxidative stress, speeding wound epithelialization, and developing more structured tissue with interlocking collagen bundles than HPRP wounds. However, further ultrasonography study is required to investigate the effect of PRP gel on cutaneous wound healing in rescued donkeys.

DOI

10.21608/ejvs.2022.143361.1348

Keywords

Cutaneous Wound Healing, donkeys, Masson’s trichrome, Oxidative Stress, Platelet-Rich Plasma Gel

Authors

First Name

Muhammad

Last Name

Sajjad

MiddleName

Talha

Affiliation

Department of Veterinary Surgery and Pet Sciences, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

Email

yazdani665@gmail.com

City

Lahore

Orcid

0000-0002-1510-5281

First Name

Hamid

Last Name

Akbar

MiddleName

-

Affiliation

Department of Veterinary Surgery and Pet Sciences,Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

Email

hamid.akbar@uvas.edu.pk

City

Lahore

Orcid

-

First Name

Muhammad

Last Name

Hayat

MiddleName

Abid

Affiliation

Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University 150030, Harbin, China

Email

abid.hayat@uvas.edu.pk

City

Harbin

Orcid

0000-0002-1109-0764

First Name

Muhammad

Last Name

Khan

MiddleName

Arif

Affiliation

Department of Veterinary Surgery and Pet Sciences, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

Email

marif@uvas.edu.pk

City

Lahore

Orcid

0000-0002-9734-3899

First Name

Muhammad

Last Name

Mushtaq

MiddleName

Hassan

Affiliation

Department of Epidemiology and Public Health, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

Email

hassan.mushtaq@uvas.edu.pk

City

Lahore

Orcid

0000-0002-6946-7931

First Name

Shehla

Last Name

Bokhari

MiddleName

Gul

Affiliation

Department of Veterinary Surgery and Pet Sciences, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

Email

shehla.gul@uvas.edu.pk

City

Lahore

Orcid

0000-0003-0360-8380

First Name

Ghulam

Last Name

Mustafa

MiddleName

-

Affiliation

Department of Veterinary Pathology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan

Email

gmustafa@uvas.edu.pk

City

Lahore

Orcid

0000-0002-8701-977X

Volume

53

Article Issue

4

Related Issue

35959

Issue Date

2022-12-01

Receive Date

2022-06-07

Publish Date

2022-12-01

Page Start

529

Page End

539

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 53_Issue 4_Pages 529-539.pdf

PDF . 1.6MB

Link

https://ejvs.journals.ekb.eg/article_254002.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=254002

Order

254,002

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

The Effect of Autologous and Homologous Platelets-Rich Plasma Gel on Cutaneous Wound Healing In Rescued Donkeys: A Comparative Study

Details

Type

Article

Created At

22 Jan 2023