The aim of this trail is to using omega-3 as a prophylactic or curative, or both on gingival injury healing in rabbits. To attain this objective has been employed 36 rabbits, were divided into 3 groups, each with 12 animals. The first group were considered as a control group, receiving normal saline for 10 days, while the second group received omega-3 for 10 days before gingival incision. The third group received omega-3 for 10 days before and continuous treatment for 10 days after the gingival incision. An incision was done in the gingival (0.5 cm)  in all groups. The group given Omega-3 for 10 days before and after the woundperforming had the superior results of the preceding therapies. It enhanced re-epithelization score and substantial increase in granulation tissue formation and angiogenesis, and decreaseinflammation scores. The histopathological section (3 days post-surgery) showed the site of the wound with re-epithelialization (score 4), granulation tissue (score 2), angiogenesis (score 3), and without inflammation (score 0), as well as 7 days post-surgery were the same scores except the score of granulation tissuewas 3.  Using omega-3 has a superior effect, especially when used for both prophylactic and treatment, and it helped to reduce inflammatory markers while improving healing indications such as re-epithelialization, granulation tissue, and angiogenesis. As a result, omega-3 can be considered a beneficial value as antimicrobial agent in the treatment of wounds and gingivitis.