E.coli infection have a great impact on poultry production since the first days after chicks hatching. We aimed in the current study to evaluate the effect of ProbChick® on the production and pathological aspect of broiler infected experimentally with E. coli. A total of 200 one-day-old chicks were divided randomly into five groups. The first group was considered a control group. The second group consumes ProbChick® with drinking water. The third group was orally inoculated with E. coli at dose 0.5 ml containing 6x108 CFU/ml. The fourth group was orally inoculated with E. coli at dose 0.5 ml containing 6x108 CFU/ml then after 7 days consume ProbChick®. The fifth group was orally inoculated with E. coli at dose 0.5 ml containing 6x108 CFU/ml and consume ProbChick® at the same day. The result showed that, ProbChick® enhance the weight gain, food conversion ratio, relative weight for kidney and liver, in contrast in groups that infected with E. coli, the ProbChick® help to reduce the effect of experimental infection with this bacterium, especially when it is give at the same day of infection, this was obtained from the improvement of the histopathological changes that were less in their severity in comparison with the group infected with E. coli only. We conclude that adding ProbChick® to broiler feed will enhance their production properties in addition, and cause noticeable reduction in the severity of lesions induced by experimental infection with pathogenic E. coli.