The AIM of the present study was to assess the efficacy of intrauterine infusion of silver nanoparticles for the treatment of endometritis in cows. The research was carried out in a dairy cow field with Iraqi breed cows. The study involved 20 cows with clinical endometritis (CE). cows were randomly allocated into two groups (G1and G2 ). Clinical endometritis were clinically diagnosed depending up on a case history, clinical signs, ultrasound and estimate polymorphoneuclear cells (PMNC) in uterine mucus. Before treatment, the involved cows in the study were subjected to the following examinations:  Color and pH of uterine mucus, Measurement of the thickness of endometrium by Ultrasonography, percentage of PMNCs in the uterine mucus, The first group (G1)  was given a 20 ml Intrauterine infusion containing 100 mg of AgNPs. The second group (G2) was left untreated. After 21 days of treatment, all of the animals were Re-examined for: Color and pH of uterine mucus, thickness of the uterine wall, and percentage of PMNCs in the uterine mucus and response rate. Results of present study revealed that the cure rate for endometritis in G1,G2 were 70% and 20%, respectively, based up on both color and pH of uterine mucus, thickness of endometrium, and percentage of PMNCs before and after treatment with a significant difference (P = <0.001). We conclude that Silver nanoparticles are very effective in treating endometritis in cows.