Axonal regeneration of the nerve is the important target to functional recovery after  the accident and peripheral nerve injury. Zinc oxide have many properties like antimicrobial activity, osteogenesis and angiogenesis, and  have  cytotoxic in different tissue in high dose. The effects on the  nerve tissues may enhance functional recovery after peripheral nerve injury. The aim of the study was to evaluate the therapeutic effects of systemic ZnO NPs following inferior alveolar nerve (IAN) crush injury. Twelve rabbits were divided two groups six rabbits in the every group treated and control, all the animal were have the injury in left side of the face following the nerve crush injuries of the IAN, the treated group receive daily intramuscularly  injections of 20 mg/kg 1 ZnO NPs. Fourteen days after induction of nerve injuries, ZnO NPs was injected over the mental foramen for the evaluation of neuronal survival. At the end of the 2 week period, histologic examination of IAN samples were performed. The microscopic evaluation showed the ability of ZnO NP to enhance the nerve regeneration in the  peripheral nerve and the high dose effect to the brain tissue.