Our study applied on 160 collected samples, from poultry and human patients. Infection percent of human skin, poultry bumble foot and human nasal swab and Diabetic foot swab were 66%  , 10% , 67.5% , 37.5%  respectively. Methicillin resistant Staph aureus (MRSA) were 83.3% and 50 % from poultry and human patient respectively, mec A gene is detected by 100% on samples of poultry and humen origin. Whereas blaZ and spa gene and also, 83.3% and 50% respectively either from human or poultry samples respectively. Methicillin Resistance S. aureus (MRSA) strains isolated from human nasal swabs was 80 % . PCR technique was used for mec A , blaZ ,and  Spa gene detection. PCR results were identical to disc diffusion method in 11 isolates out of 15 ( 73 %) isolates from poultry and human samples.